CD16 Mouse anti-Human, PerCP-Cy5.5, Clone: 3G8, BD

Mouse Monoclonal Antibody

Supplier:  BD Biosciences 560717

Catalog No. BDB560717

Description

The 3G8 monoclonal antibody specifically binds to the 50-65 kDa transmembrane form of the IgG Fc Receptor (FcγRIII), a human NK cell-associated antigen. CD16 is expressed on NK cells as well as macrophages and granulocytes. Reports indicate that CD16 plays a role in signal transduction and NK cell activation. The 3G8 antibody blocks the binding of soluble immune complexes to granulocytes. The 3G8 antibody is reported (Vossebeld et al., 1997) to increase intracellular calcium levels in human neutrophils by interacting with bothFcγRIIa and FcγRIIIb molecules. This antibody has also been reported to induce homotypic neutrophil aggregation.

This clone also cross-reacts with a subset of peripheral blood lymphocytes and monocytes, but not granulocytes, of baboon and both rhesus and cynomologus macaque monkeys. Multi-color analysis reveals that the distribution on lymphocytes is similar to that found in human studies with the majority of CD16-positive lymphocytes being both CD3 and CD20 negative.

Specifications

• Antigen: CD16
• Classification: Monoclonal
• Conjugate: PerCP-Cyanine5.5
• Formulation: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
• Immunogen: Human polymorphonuclear leukocytes
• Quantity: 50 Tests
• Research Discipline: Immunology
• Target Species: Baboon, Cynomolgus, Human, Rhesus Monkey
• Isotype: IgG1 κ

• Applications: Flow Cytometry
• Clone: 3G8
• Description: FcRIII; Fc-gamma RIII; FCG3; FCGR3; FCGRIII; FcγRIII; IGFR3
• Host Species: Mouse
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.