Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More
  1. Home
  2. Products
  3. Antibodies
  4. Primary Antibodies
  5. All Primary Antibodies
  6. BDB560076

CD22 (pY822) Mouse, PE, Clone: 12a/CD22, BD

Catalog No. BDB560076 Shop All BD Biosciences Products
Change view
Click to view available options
Quantity:
50 Tests
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Catalog No. Quantity
BDB560076 50 Tests
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
Catalog No. BDB560076 Supplier BD Biosciences Supplier No. 560076
Add to Cart
Add to Cart

Description

Mouse Monoclonal Antibody

CD22 is a member of the immunoglobulin (Ig) superfamily that contains several extracellular Ig domains and cytoplasmic I mmunoreceptor T yrosine-based I nhibitory a has only 5. CD22 b is the predominant isoform in human cells, at both the mRNA and protein levels. Ligands for the extracellular domains of CD22 are sialic acid-linked glycoproteins on the B cell surface. The intracellular ITIM motifs are substrates of src family tyrosine kinases. Signal transduction is initiated by the phosphorylation of tyrosines in the ITIMs of CD22 upon ligation of the B cell receptor (BCR). Tyrosine phosphorylation in ITIM3 of human CD22 or ITIM2 of mouse CD22 is required for recruitment of the protein tyrosine phosphatase PTP1C (SHP-1), which may terminate signal transduction by CD22 and by co-localized receptors, such as the BCR. Studies of mice deficient for CD22 suggest that this adhesion molecule generates both negative and positive regulatory signals that affect B cell development and responsiveness.

The 12a/CD22 monoclonal antibody recognizes the phosphorylated tyrosine 822 (pY822) in ITIM3 of human CD22 b . The equivalent site in human CD22 a is pY645. The orthologous phosphorylation site in the predominant isoform of mouse CD22 is pY837, which is in ITIM2.

We recommend the PE conjugate of mAb 12a/CD22, Cat. No. 560076, for staining both human and mouse leukocytes.

TRUSTED_SUSTAINABILITY

Specifications

Antigen CD22 (pY822)
Applications Flow Cytometry
Classification Monoclonal
Clone 12a/CD22
Conjugate PE
Description Human BL-CAM, Siglec-2, or Leu-14 (pY822); Mouse Lyb-8 (pY837)
Formulation Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Host Species Mouse
Immunogen Phosphorylated Mouse CD22 Peptide
Purification Method Affinity Purified
Quantity 50 Tests
Regulatory Status RUO
Primary or Secondary Primary
Target Species Human, Mouse
Content And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Isotype IgG1 κ
Show More Show Less
Product Title
Select an issue

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.