CD247 (pY142) Mouse anti-Human, Unlabeled, Clone: K25-407.69, BD

Mouse Monoclonal Antibody

Supplier:  BD Biosciences 558402

Catalog No. BDB558402

Description

The T cell receptor (TCR), expressed by thymocytes and T lymphocytes, is a multi-component cell-surface complex responsible for recognizing antigen in the context of MHC molecules. The antigen-specific binding component of the TCR, Ti, is a heterodimer of the variable Ig-like subunits a and b or g and d . Ti is non-covalently associated with an invariant set of molecules referred to as the CD3 polypeptides, g, d, e, and ζ ) was named CD247 at the 7th Human Leukocyte Differentiation Antigens Workshop. CD3 appears early in thymocyte differentiation and remains expressed on all mature T lymphocytes. After antigen recognition by the TCR, CD3 z is the primary intracellular signal transducing subunit. It contains three ITAMs ( I mmunoreceptor T yrosine-based A ctivation M otifs), each of which contains a pair of tyrosine residues that are phosphorylated by Lck and Fyn and are required for signal propagation. The molecular weight of CD3 z is 16 kDa, and it is also observed as 32-kDa homodimers or as heterodimers with the g chain of Fc receptors. Upon phosphorylation, the CD3 z monomer undergoes an apparent shift in electrophoretic mobility up to 21 kDa.

The K25-407.69 monoclonal antibody recognizes the phosphorylated tyrosine 142 (pY142) in the third ITAM domain of human CD3 z (CD247) .

Specifications

• Antigen: CD247 (pY142)
• Classification: Monoclonal
• Concentration: 0.5mg/mL
• Description: CD3ζ
• Host Species: Mouse
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C

• Applications: Western Blot
• Clone: K25-407.69
• Conjugate: Unconjugated
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Immunogen: Mouse NKG2D protein
• Quantity: 0.1 mg
• Research Discipline: Immunology
• Target Species: Human
• Isotype: IgG2a κ

For Research Use Only.