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Description
The T cell receptor (TCR), expressed by thymocytes and T lymphocytes, is a multi-component cell-surface complex responsible for recognizing antigen in the context of MHC molecules. The antigen-specific binding component of the TCR, Ti, is a heterodimer of the variable Ig-like subunits a and b or g and d . Ti is non-covalently associated with an invariant set of molecules referred to as the CD3 polypeptides, g, d, e, and ζ ) was named CD247 at the 7th Human Leukocyte Differentiation Antigens Workshop. CD3 appears early in thymocyte differentiation and remains expressed on all mature T lymphocytes. After antigen recognition by the TCR, CD3 z is the primary intracellular signal transducing subunit. It contains three ITAMs ( I mmunoreceptor T yrosine-based A ctivation M otifs), each of which contains a pair of tyrosine residues that are phosphorylated by Lck and Fyn and are required for signal propagation. The molecular weight of CD3 z is 16 kDa, and it is also observed as 32-kDa homodimers or as heterodimers with the g chain of Fc receptors. Upon phosphorylation, the CD3 z monomer undergoes an apparent shift in electrophoretic mobility up to 21 kDa.
The K25-407.69 monoclonal antibody recognizes the phosphorylated tyrosine 142 (pY142) in the third ITAM domain of human CD3 z (CD247) .
Specifications
Specifications
| Antigen | CD247 (pY142) |
| Applications | Western Blot |
| Classification | Monoclonal |
| Clone | K25-407.69 |
| Concentration | 0.5mg/mL |
| Conjugate | Unconjugated |
| Description | CD3ζ |
| Formulation | Aqueous buffered solution containing ≤0.09% sodium azide. |
| Host Species | Mouse |
| Immunogen | Mouse NKG2D protein |
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For Research Use Only.
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