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CD25 Rat anti-Mouse, Brilliant Violet 421, Clone: 7D4, BD

Rat Monoclonal Antibody

Manufacturer:  BD Biosciences 564571

Catalog No. BDB564571


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Description

Description

The 7D4 monoclonal antibody specifically binds to CD25, the low affinity IL-2 Receptor (IL-2R α , p55) expressed on activated T and B lymphocytes from all mouse strains tested. IL-2R α by itself is not a signaling receptor. However, it can combine with IL-2 Receptor β (CD122) and γ(CD132) chains to form high-affinity, signaling receptor complexes for IL-2. Resting T and B lymphocytes and resting and activated NK cells do not express IL-2R α . CD25 is transiently expressed at a low level during normal B-cell development in the bone marrow on the CD45R/B220low TdT- sIg- Pre-B/Pre-B-II and CD45R/B220low TdT- sIgM+ sIgD- immature B stages, but not on the CD45R/B220low TdT+ sIg- Pro-B/Pre-B-I stage nor on CD45R/B220high TdTsIgM+ sIgD+ mature B cells. It is expressed at a higher level during a very early stage of T-cell development in fetal and adult thymus. Peripheral CD25+ CD4+ T lymphocytes called regulatory T (Treg) cells are involved in the maintenance of self-tolerance. It has also been reported that dendritic cells express CD25, recognized by mAb 7D4. The 3C7 antibody recognizes an epitope of CD25 which is distinct from those recognized by mAbs 7D4 and PC61, and it blocks binding of IL-2 to CD25.

The antibody was conjugated to BD Horizon BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates.

Specifications

Specifications

CD25
7D4
Brilliant Violet 421
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Rat
IgM, κ
50μg
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Monoclonal
Flow Cytometry
0.2mg/mL
IL-2 Receptor α chain, p55
Affinity Purified
IL-2-dependent BALB/c mouse helper T-cell clone HT-2
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
RUO
Primary
Murine
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