CD25 Rat anti-Mouse, Brilliant Violet 786, Clone: 3C7, BD
Rat Monoclonal Antibody
Manufacturer: BD Biosciences 564368
The 3C7 monoclo nal antibody specifically binds to CD25, the low affinity IL-2 Receptor (IL-2Rα, p55) expressed on activated T and B lymphocytes from all mouse strains tested. IL-2Rα by itself is not a signaling receptor. However, it can combine with IL-2 Receptor β (CD122) and γc (CD132) chains to form high-affinity, signaling receptor complexes for IL-2. Resting T and B lymphocytes and resting and activated NK cells do not express IL-2Rα. CD25 is transiently expressed at a low level during normal B-cell development in th e bone marrow on the CD45R/B220low TdT- sIg- Pre-B/Pre-B-II and CD45R/B220low TdT- sIgM+ sIgD- immature B stages, but not on the CD45R/B220low TdT+ sIg- Pro-B/Pre B-I stage nor on CD45R/B220high TdTsIgM+ sIgD+ mature B cells. It is expressed at a higher level during a very early stage of T-cell development in fetal and adult thymus. Peripheral CD25+ CD4+ T lymphocytes called regulatory T (Treg) cells are involved in the maintenance of self-tolerance. It has also been reported that dendritic cells express CD25, recognized by mAb 7D4, another CD25-specific antibody. The 3C7 antibody recognizes an epitope of CD25 which is distinct from those recognized by other CD25-specific mAbs, 7D4 and PC61. 3C7 blocks the binding of IL-2 to CD25.
The antibody was conjugated to BD Horizon BV786 which is part of the BD Horizon Brilliant Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 786-nm. BD Horizon BV786 can be excited by the violet laser and detected in a filter used to detect Cy7-like dyes (eg, 780/60-nm filter).
|Brilliant Violet 786|
|Aqueous buffered solution containing BSA and ≤0.09% sodium azide.|
|Interleukin-2 receptor alpha chain; IL-2RA; IL-2Rα; Il2ra; IL-2R p55|
|IL-2-dependent BALB/c mouse cell line|
|Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.|
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