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CD25 Rat anti-Mouse, Brilliant Violet 786, Clone: PC61, BD

Rat Monoclonal Antibody

Manufacturer:  BD Biosciences 564023

Catalog No. BDB564023


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Description

Description

The PC61 antibody reacts with CD25, the low-affinity IL-2 Receptor α chain (IL-2Rα, p55) expressed on activated T and B lymphocytes from all mouse strains tested. IL-2Rα by itself is not a signaling receptor. However, it can combine with IL-2 Receptor β (CD122) and γc (CD132) chains to form high-affinity, signaling receptor complexes for IL-2. Resting T and B lymphocytes and resting and activated NK cells do not express IL-2Rα. CD25 is transiently expressed at a low level during normal B-cell development in the bone marrow on the CD45R/B220low TdT- sIg- Pre-B/Pre-B-II and CD45R/B220low TdT- sIgM+ sIgD- immature B stages, but not on the CD45R/B220low TdT+ sIg- Pro-B/Pre-B-I stage nor on CD45R/B220high TdT- sIgM+ sIgD+ mature B cells. It is expressed at a higher level during a very early stage of T-cell development in fetal and adult thymus. Peripheral CD25+CD4+ lymphocytes called regulatory T (Treg) cells are involved in the maintenance of self-tolerance. It has also been reported that dendritic cells express CD25, recognized by mAb 7D4 (Cat. No. 553068). The PC61 antibody recognizes an epitope of CD25 which is distinct from the IL-2 binding site and from those recognized by mAbs 3C7 (Cat. No. 557364) and 7D4 (Cat. No. 553068). It blocks binding of IL-2 to CD25, presumably by inducing a conformational change in CD25.

The antibody was conjugated to BD Horizon™ BV786 which is part of the BD Horizon Brilliant Violet™ family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 786-nm. BD Horizon BV786 can be excited by the violet laser and detected in a filter used to detect Cy7™-like dyes (eg, 780/60-nm filter).

Specifications

Specifications

CD25
PC61
Brilliant Violet 786
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Rat
IgG1, λ
RUO
Primary
Murine
Flow Cytometry
0.2mg/mL
Interleukin-2 receptor alpha chain; IL-2RA; IL-2Rα; Il2ra; IL-2R p55
Affinity Purified
IL-2-dependent cytolytic mouse T-cell clone B6.1
50μg
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Monoclonal
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