CD25 Mouse anti-Human, BUV395, Clone: 2A3, BD

Catalog No.	Quantity
BDB564034	100 Tests

Catalog No. BDB564034 Supplier BD Biosciences Supplier No. 564034

Description

Mouse Monoclonal Antibody

The 2A3 monoclonal antibody specifically binds to human CD25, the low-affinity alpha subunit of the Interleukin-2 Receptor (IL-2R α). CD25 associates with CD122 (IL-2R β chain) and CD132 (common γchain or γc) to form the high-affinity signal-transducing IL-2R complex. CD25 is expressed by a subset of peripheral blood lymphocytes including CD4+CD25+ natural regulatory T cells. CD25 antigen density increases on activated T cells including phytohemagglutinin (PHA)-, concanavalin A (Con A)-, and CD3-activated T lymphocytes. High levels of CD25 can also be expressed by T lymphocytes from mixed lymphocyte cultures and by human T-lymphocyte leukemia virus (HTLV)-infected T-lymphocyte leukemia lines, for example, HUT-102. Recombinant IL-2 blocks the binding of the 2A3 antibody to PHA-activated T lymphocytes .

The antibody was conjugated to BD Horizon™ BUV395 which has been exclusively developed by BD Biosciences as an optimal dye for use on a 355nm laser equipped instrument. With an Ex Max at 348nm and an Em Max at 395nm, this dye has virtually no spillover into any other detector. BD Horizon™ BUV395 can be excited with a 355nm laser and detected with a 379/28 filter.

Specifications

• Antigen: CD25
• Applications: Flow Cytometry
• Classification: Monoclonal
• Clone: 2A3
• Conjugate: Brilliant Ultraviolet 395
• Description: IL-2R; IL2RA; IL-2Rα; TCGFR; TAC antigen; p55
• Formulation: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
• Host Species: Mouse
• Immunogen: Human Phytohemagglutinin-activated T Cells
• Purification Method: Affinity Purified
• Quantity: 100 Tests
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Human
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
• Isotype: IgG1 κ