Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

CD25 Mouse anti-Human, BUV395, Clone: 2A3, BD

Mouse Monoclonal Antibody

Manufacturer:  BD Biosciences 564034

Catalog No. BDB564034


Add to cart

Description

Description

The 2A3 monoclonal antibody specifically binds to human CD25, the low-affinity alpha subunit of the Interleukin-2 Receptor (IL-2R α). CD25 associates with CD122 (IL-2R β chain) and CD132 (common γchain or γc) to form the high-affinity signal-transducing IL-2R complex. CD25 is expressed by a subset of peripheral blood lymphocytes including CD4+CD25+ natural regulatory T cells. CD25 antigen density increases on activated T cells including phytohemagglutinin (PHA)-, concanavalin A (Con A)-, and CD3-activated T lymphocytes. High levels of CD25 can also be expressed by T lymphocytes from mixed lymphocyte cultures and by human T-lymphocyte leukemia virus (HTLV)-infected T-lymphocyte leukemia lines, for example, HUT-102. Recombinant IL-2 blocks the binding of the 2A3 antibody to PHA-activated T lymphocytes .

The antibody was conjugated to BD Horizon™ BUV395 which has been exclusively developed by BD Biosciences as an optimal dye for use on a 355nm laser equipped instrument. With an Ex Max at 348nm and an Em Max at 395nm, this dye has virtually no spillover into any other detector. BD Horizon™ BUV395 can be excited with a 355nm laser and detected with a 379/28 filter.

Specifications

Specifications

CD25
2A3
IL-2R; IL2RA; IL-2Rα; TCGFR; TAC antigen; p55
Affinity Purified
Human Phytohemagglutinin-activated T Cells
100 Tests
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Monoclonal
Flow Cytometry
Brilliant Ultraviolet 395
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Murine
IgG1, κ
RUO
Primary
Human
Documents
Provide Content Correction

We continue to work to improve your shopping experience and your feedback regarding this content is very important to us. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Cancel Submit