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CD25 Mouse anti-Human, Brilliant Violet 711, Clone: 2A3, BD

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Description

Mouse Monoclonal Antibody

The 2A3 monoclonal antibody specifically binds to human CD25, the low-affinity alpha subunit of the Interleukin-2 Receptor (IL-2R α ). CD25 associates with CD122 (IL-2R β chain) and CD132 (common γchain or γc) to form the high-affinity signal-transducing IL-2R complex. CD25 is expressed by a subset of peripheral blood lymphocytes including CD4+CD25+ natural regulatory T cells. CD25 antigen density increases on activated T cells including phytohemagglutinin (PHA)-, concanavalin A (Con A)-, and CD3-activated T lymphocytes. High levels of CD25 can also be expressed by T lymphocytes from mixed lymphocyte cultures and by human T-lymphocyte leukemia virus (HTLV)-infected T-lymphocyte leukemia lines, for example, HUT-102. Recombinant IL-2 blocks the binding of the 2A3 antibody to PHA-activated T lymphocytes.

The antibody was conjugated to BD Horizon™ BV711 which is part of the BD Horizon™ Brilliant Violet™ family of dyes. This dye is a tandem fluorochrome of BD Horizon™ BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 711-nm. BD Horizon™ BV711 can be excited by the violet laser and detected in a filter used to detect Cy™5.5 / Alexa Fluor™ 700-like dyes (eg, 712/20-nm filter). Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy™5.5 detectors. However, the spillover can be corrected through compensation as with any other dye combination.

TRUSTED_SUSTAINABILITY

Specifications

Antigen CD25
Applications Flow Cytometry
Classification Monoclonal
Clone 2A3
Conjugate Brilliant Violet 711
Description IL-2R; IL2RA; IL-2Rα; TCGFR; TAC antigen; p55
Formulation Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Host Species Mouse
Immunogen Human Phytohemagglutinin-activated T Cells
Purification Method Affinity Purified
Quantity 50 Tests
Regulatory Status RUO
Primary or Secondary Primary
Target Species Human
Content And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Isotype IgG1 κ
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Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov
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