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  6. CD25 Mouse anti-Human, Brilliant Violet 421, Clone: M-A251, BD

CD25 Mouse anti-Human, Brilliant Violet 421, Clone: M-A251, BD

Catalog No. BDB562442 Shop All BD Biosciences Products
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Description

Mouse Monoclonal Antibody

The M-A251 monoclonal antibody specifically binds to the 55 kDa type I transmembrane glycoprotein known as the low-affinity interleukin-2 receptor alpha chain subunit (IL-2Rα). CD25 is expressed on regulatory T cells and on activated lymphocytes (T and B) and monocytes. It associates with the IL-2Rβ/CD122 and the IL-2Rγ/CD132 receptor chains to form the high-affinity IL-2R complex. CD25 expression on T and B lymphocytes is upregulated by antigenic or mitogenic stimulation. Soluble CD25/IL-2Rα is produced as a consequence of lymphocyte stimulation and is found in biological fluids following inflammatory responses. The antibody was conjugated to Brilliant Violet 421 and has been developed in collaboration with Sirigen. With an Ex Max of 407-nm and Em Max at 421-nm, Brilliant Violet 421 can be excited by the violet laser and detected in the standard Pacific Blue filter set (eg, 450/50-nm filter). Brilliant Violet 421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates.

Host Species: Mouse
Clone: M-A251
Isotype: IgG1, κ
Species Reactivity: Human

Flow Cytometry

TRUSTED_SUSTAINABILITY

Specifications

Antigen CD25
Applications Flow Cytometry
Classification Monoclonal
Clone M-A251
Conjugate Brilliant Violet 421
Description IL-2R; IL2RA; IL-2Rα; TCGFR; TAC antigen; p55
Formulation Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Host Species Mouse
Purification Method Affinity Purified
Quantity 100 Tests
Regulatory Status RUO
Primary or Secondary Primary
Target Species Human
Content And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Isotype IgG1 κ
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Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only.

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