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CD25 Mouse anti-Human, Brilliant Violet 510, Clone: M-A251, BD

Mouse Monoclonal Antibody

Manufacturer:  BD Biosciences 563352

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Catalog No. BDB563352

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The M-A251 monoclonal antibody specifically binds to the 55 kDa type I transmembrane glycoprotein known as the low-affinity interleukin-2 receptor alpha chain subunit (IL-2Rα). CD25 is expressed on regulatory T cells and on activated lymphocytes (T and B) and monocytes. It associates with the IL-2Rβ/CD122 and the IL-2Rγ/CD132 receptor chains to form the high-affinity IL-2R complex. CD25 expression on T and B lymphocytes is upregulated by antigenic or mitogenic stimulation. Soluble CD25/IL-2Rα is produced as a consequence of lymphocyte stimulation and is found in biological fluids following inflammatory responses.

The antibody was conjugated to BD Horizon™ BV510 which is part of the BD Horizon™ Brilliant Violet™ family of dyes. With an Ex Max of 405-nm and Em Max at 510-nm, BD Horizon™ BV510 can be excited by the violet laser and detected in the BD Horizon™ V500 (525/50-nm) filter set. BD Horizon™ BV510 conjugates are useful for the detection of dim markers off the violet laser.



IL-2R; IL2RA; IL-2Rα; TCGFR; TAC antigen; p55
Affinity Purified
IgG1, κ
Flow Cytometry
Brilliant Violet 510
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
100 Tests
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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