CD25 Mouse anti-Human, V450, Clone: M-A251, BD

Mouse Monoclonal Antibody

Supplier:  BD Biosciences 560355

Catalog No. BDB560355

Description

Reacts with CD25, a 55kDa glycoprotein known as the low-affinity interleukin-2 receptor (IL-2R). CD25 is expressed on activated lymphocytes (T and B) and monocytes, and associates with the p75 high-affinity receptor chain to form the IL-2R complex. The CD25 molecule reveals three epitope regions: A, B, and C. M-A251 antibody recognizes epitope region B. CD25 expression on lymphocytes is upregulated by anti-CD3 or PHA stimulation. Soluble IL-2R is produced following inflammatory responses. The antibody is conjugated to BD Horizon™ V450, which has been developed for use in multicolor flow cytometry experiments and is available exclusively from BD Biosciences. It is excited by the Violet laser Ex max of 406nm and has an Em Max at 450nm. Conjugates with BD Horizon™ V450 can be used in place of Pacific Blue™ conjugates.

Host Species: Mouse
Clone: M-A251
Isotype: IgG1 κ
Species Reactivity: Human

Flow Cytometry, Functional Assay

Specifications

• Antigen: CD25
• Classification: Monoclonal
• Conjugate: Violet Fluor 450 (V450)
• Formulation: Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

• Applications: Flow Cytometry
• Clone: M-A251
• Description: IL-2R; IL2RA; IL-2Rα; TCGFR; TAC antigen; p55
• Host Species: Mouse
• Quantity: 120 Tests
• Research Discipline: Immunology
• Target Species: Human
• Isotype: IgG1 κ

For Research Use Only.