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CD253 Rat anti-Mouse, Unlabeled, Clone: N2B2, BD

Catalog No. BDB550320 Shop All BD Biosciences Products
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BDB550320 0.1 mg
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Catalog No. BDB550320 Supplier BD Biosciences Supplier No. 550320
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Description

Rat Monoclonal Antibody

The N2B2 antibody reacts with mouse TRAIL (TNF-Related Apoptosis-Inducing Ligand, also known as APO-2 ligand or Ly-81), a 40-kDa type-II transmembrane protein of the TNF family. This antibody recognizes mouse Trail -transfected 2PK-3 cells, but not the parental cell line. TRAIL selectively induces apoptosis in various transformed cells, but not in normal cells. Five receptors have been identified for human TRAIL, whereas only one receptor has been described for the mouse counterpart thus far. The pattern of expression of mouse TRAIL is controversial. TRAIL has been detected, with a rabbit polyclonal antibody, on the surface of activated CD4+ and CD8+ T lymphocytes, as well as on CD45R/B220+ lymphocytes, and transformed T- and B-cell lines. Similar results were obtained by western blot and mRNA analyses. However, these results could not be reproduced with the N2B2 mAb. TRAIL expression, detected with the N2B2 mAb, is induced on CD3- NK1.1+ cells after stimulation with IL-2 or IL-15. However, neither freshly isolated nor activated T cells showed TRAIL expression on the cell surface. Furthermore, TRAIL mediates NK-cell cytotoxicity, which is blocked by the N2B2 mAb. TRAIL has been renamed as CD253 recently.

TRUSTED_SUSTAINABILITY

Specifications

Antigen CD253
Applications Flow Cytometry
Classification Monoclonal
Clone N2B2
Concentration 0.5mg/mL
Conjugate Unconjugated
Description TRAIL, APO-2L, Ly-81, TL2
Formulation Aqueous buffered solution containing ≤0.09% sodium azide.
Host Species Rat
Immunogen Mouse Trail-transfected 2PK-3 cells
Purification Method Affinity Purified
Quantity 0.1 mg
Regulatory Status RUO
Research Discipline Immunology
Primary or Secondary Primary
Target Species Mouse
Content And Storage Store undiluted at 4°C
Isotype IgG2a κ
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Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only.

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