CD279 (PD-1) Mouse anti-Human, Brilliant Violet 421, Clone: MIH4, BD

Catalog No.	Quantity
BDB564323	100 Tests

Catalog No. BDB564323 Supplier BD Biosciences Supplier No. 564323

Description

Mouse Monoclonal Antibody

The MIH4 monoclonal antibody specifically binds to the program death 1 (PD-1) receptor CD279, a member of the Ig superfamily. CD279 is an immunoregulatory receptor expressed on activated T cells, B cells and myeloid cells. Mice deficient in CD279 show a breakdown of peripheral tolerance and manifest multiple autoimmune symptoms. It contains an immunoreceptor tyrosine-based inhibitory motif (ITIM) in the cytoplasmic region. PD-L1 and PD-L2 are ligands of CD279 and are members of the B7 gene family. Interaction of CD279:PD-Ligands results in inhibition of T cell proliferation and cytokine secretion. Reports suggest that the B7/CTLA-4 pathway functions primarily to attenuate, limit, and/or terminate naοve T-cell activation in secondary lymphoid organs. The PD-ligand:CD279 pathway, on the other hand, may primarily attenuate, limit, and/or terminate T-, B-, and myeloid cell activation/effector function at sites of inflammation in the periphery.

The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates.

Specifications

• Antigen: CD279 (PD-1)
• Applications: Flow Cytometry
• Classification: Monoclonal
• Clone: MIH4
• Conjugate: Brilliant Violet 421
• Description: PD-1; hPD-1; PD1; PDCD1; Programmed cell death 1; SLEB2
• Formulation: Aqueous buffered solution containing BSA and ≤0.09% sodium azide
• Host Species: Mouse
• Immunogen: Human PD-1 Transfected Cell Line
• Purification Method: Affinity Purified
• Quantity: 100 Tests
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Human
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
• Isotype: IgG1 κ