CD28 Mouse anti-Human, NA/LE, Unlabeled, Clone: CD28.2, BD

Mouse Monoclonal Antibody

Supplier:  BD Biosciences 555725

Catalog No. BDB555725

Description

The CD28.2 monoclonal antibody specifically binds to CD28, a 44 kDa homodimeric transmembrane glycoprotein present on most mature T cells, thymocytes and plasma cells. CD28 is a costimulatory receptor that binds CD80 and CD86 as ligands and plays a very important role in T cell-B cell interactions. It has been suggested that CD28 initiates and regulates a separate and distinct signal transduction pathway from those stimulated by the TCR complex. Additionally, it has been reported that CD28 antibody clones vary in their ability to stimulate T cells to produce IL-2 and increase intracellular Ca2+ concentration. This finding suggests the existence of functionally distinct subregions on the CD28 molecule. CD28.2 has been demonstrated to bind to the same molecule as clone L293, another CD28 mAb, and has been reported to induce Ca2+ influx in Jurkat T cells.

Specifications

• Antigen: CD28
• Classification: Monoclonal
• Concentration: 1.0mg/mL
• Description: CD28 antigen; T44; Tp44; TP44
• Host Species: Mouse
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C

• Applications: Flow Cytometry
• Clone: CD28.2
• Conjugate: Unconjugated
• Formulation: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered. Endotoxin level is ≤0.01 EU/μg (≤0.001 ng/μg) of protein as determined by the LAL assay.
• Immunogen: Human CD28 Transfected Cell Line
• Quantity: 0.5 mg
• Research Discipline: Immunology
• Target Species: Human
• Isotype: IgG1 κ

For Research Use Only.