CD28 Mouse anti-Rat, NA/LE, Unlabeled, Clone: JJ316, BD

Catalog No.	Quantity
BDB554992	0.5 mg

Catalog No. BDB554992 Supplier BD Biosciences Supplier No. 554992

Description

Mouse Monoclonal Antibody

The JJ316 antibody reacts with CD28 on virtually all T lymphocytes bearing αβT-cell receptors (TCR), on most γδTCR-bearing T cells, and on a subset of NK cells. In the thymus, CD28 expression is developmentally regulated during the maturation of αβTCR-bearing T cells. CD28 is a costimulatory receptor required for activation of T cells; its ligands include CD80 (B7-1) and CD86 (B7-2). Soluble JJ316 enhances the proliferative responses and IL-2 production by T cells activated by anti-aß-TCR mAb R73 (Cat. no. 554910). Furthermore, at high concentrations, cross-linked JJ316 mAb stimulates resting CD4+ and CD8+T cells to proliferate, secrete IL-2, express activation markers, and gain CTL activity in the absence of signaling through the TCR. In vivo administration of mAb JJ316 results in blastogenic transformation of peripheral CD4+ cells and dramatic lymphocytosis. The alternate anti-rat CD28 mAb JJ319 (Cat. no. 554993) cannot directly activate T cells, although it has in vitro costimulatory activity.

Specifications

• Antigen: CD28
• Applications: Flow Cytometry
• Classification: Monoclonal
• Clone: JJ316
• Concentration: 1.0mg/mL
• Conjugate: Unconjugated
• Formulation: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered. Endotoxin level is ≤0.01 EU/μg (≤0.001 ng/μg) of protein as determined by the LAL assay.
• Host Species: Mouse
• Immunogen: Rat Cd28-transfected BALB/c mouse A20J B lymphoma
• Purification Method: Affinity Purified
• Quantity: 0.5 mg
• Regulatory Status: RUO
• Research Discipline: Immunology
• Primary or Secondary: Primary
• Target Species: Rat
• Content And Storage: Store undiluted at 4°C
• Isotype: IgG1 κ

For Research Use Only.