CD29 Mouse anti-Human, NA/LE, Unlabeled, Clone: HUTS-21, BD

Mouse Monoclonal Antibody

Supplier:  BD Biosciences 556047

Catalog No. BDB556047

Description

Reacts with the 130kDa integrin β1 subunit, CD29, that is expressed following cell activation as a heterodimeric complex with one of nine distinct α subunits, comprising the very late activation antigen (VLA) subfamily of adhesion receptors. The HUTS-21 β1 epitope is weakly expressed on resting lymphocytes, but is upregulated by TPA, Ca++ ionophore, or another CD29 antibody, TS2/16. This upregulation is Mn++ dependent and not supported by other divalent cations such as Ca++ and/or Mg++. The VLA receptors have a broad cellular distribution and interact with ligands like VCAm-1 and MAdCAM-1 during cell adhesion. They also interact with extra-cellular matrices including collagen, laminin, fibronectin and others. HUTS-21 antibody enhances adherence of TPA-activated lymphocytes to fibronectin. This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

Specifications

• Antigen: CD29
• Classification: Monoclonal
• Concentration: 1.0mg/mL
• Description: Integrin β1 Chain
• Host Species: Mouse
• Quantity: 0.5 mg
• Research Discipline: Immunology
• Target Species: Human
• Isotype: IgG2a κ

• Applications: Flow Cytometry
• Clone: HUTS-21
• Conjugate: Unconjugated
• Formulation: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered. Endotoxin level is ≤0.01 EU/μg (≤0.001 ng/μg) of protein as determined by the LAL assay.
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C

For Research Use Only.