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CD3 Mouse anti-Human, BB515, Clone: HIT3a, BD

Catalog No. BDB565101 Shop All BD Biosciences Products
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BDB565100 100 Tests
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Catalog No. BDB565101 Supplier BD Biosciences Supplier No. 565101
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Description

Mouse Monoclonal Antibody

The HIT3a monoclonal antibody specifically binds to the human CD3 ε-chain, a 20 kDa subunit of the CD3/T cell antigen receptor complex found on 70-80% of normal human peripheral blood lymphocytes and 60-85% of thymocytes. Studies from the HLDA Workshop show that this antibody can be mitogenic for T lymphocytes. The CD3 complex plays a role in signal transduction during antigen recognition by the T cell receptor. HIT3a antibody does not stain intracellular CD3 unlike the other CD3-specific clone, UCHT1 (Cat. No. 555330/550368).

The antibody was conjugated to BD Horizon BB515 which was developed exclusively by BD Biosciences. With an excitation max of 490nm and an emission max of 515nm, BD Horizon BB515 can be excited by the 488nm laser and detected in a standard FITC set (eg, 530/30-nm filter). This dye provides a much brighter alternative to FITC with less spillover into the PE detector.

TRUSTED_SUSTAINABILITY

Specifications

Antigen CD3
Applications Flow Cytometry
Classification Monoclonal
Clone HIT3a
Conjugate Brilliant Blue 515 (BB515)
Description CD3E; T3E; TCRE; cd 3; cd-3; cd3; CD3-epsilon; 916
Formulation Aqueous buffered solution containing ≤0.09% sodium azide
Host Species Mouse
Purification Method Affinity Purified
Quantity 25 Tests
Regulatory Status RUO
Primary or Secondary Primary
Target Species Human
Content And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Isotype IgG2a κ
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For Research Use Only.

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