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CD3 Mouse anti-Human, BB515, Clone: HIT3a, BD

Mouse Monoclonal Antibody

Manufacturer:  BD Biosciences 565101

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Catalog No. BDB565101


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Description

Description

The HIT3a monoclonal antibody specifically binds to the human CD3 ε-chain, a 20 kDa subunit of the CD3/T cell antigen receptor complex found on 70-80% of normal human peripheral blood lymphocytes and 60-85% of thymocytes. Studies from the HLDA Workshop show that this antibody can be mitogenic for T lymphocytes. The CD3 complex plays a role in signal transduction during antigen recognition by the T cell receptor. HIT3a antibody does not stain intracellular CD3 unlike the other CD3-specific clone, UCHT1 (Cat. No. 555330/550368).

The antibody was conjugated to BD Horizon BB515 which was developed exclusively by BD Biosciences. With an excitation max of 490nm and an emission max of 515nm, BD Horizon BB515 can be excited by the 488nm laser and detected in a standard FITC set (eg, 530/30-nm filter). This dye provides a much brighter alternative to FITC with less spillover into the PE detector.

Specifications

Specifications

CD3
HIT3a
CD3E; T3E; TCRE; cd 3; cd-3; cd3; CD3-epsilon; 916
Affinity purified
IgG2a, κ
RUO
Primary
Human
Flow Cytometry
BB515
Aqueous buffered solution containing ≤0.09% sodium azide
Murine
25 Tests
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Monoclonal
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