CD3 Mouse anti-Human, BB515, Clone: UCHT1, BD

Catalog No.	Quantity
BDB564466	25 Tests
BDB564465	100 Tests

Catalog No. BDB564466 Supplier BD Biosciences Supplier No. 564466

Description

Mouse Monoclonal Antibody

The UCHT1 monoclonal antibody specifically binds to the human CD3 ε -chain, a 20-kDa subunit of the CD3/T cell antigen receptor complex. CD3 ε is expressed on 70-80% of normal human peripheral blood lymphocytes and 60-85% of thymocytes. Studies from the HLDA Workshop show that this antibody is mitogenic for CD3 ε -positive cells when used in conjunction with costimulatory agents such as pokeweed mitogen or anti-CD28 antibody. CD3 plays a central role in signal transduction during antigen recognition. The UCHT1 antibody stains both surface and intracellular CD3 ε unlike the other CD3 clone, HIT3a, that stains only extracellular CD3 ε .

The antibody was conjugated to BD Horizon™ BB515 which was developed exclusively by BD Biosciences. With an excitation max of 490nm and an emission max of 515nm, BD Horizon BB515 can be excited by the 488nm laser and detected in a standard FITC set (e.g. 530/30-nm filter). This dye provides a much brighter alternative to FITC with less spillover into the PE detector.

Specifications

• Antigen: CD3
• Applications: Flow Cytometry
• Classification: Monoclonal
• Clone: UCHT1
• Conjugate: Brilliant Blue 515 (BB515)
• Description: CD3e; CD3E; T3E; TCRE; T-cell surface antigen T3/Leu-4 epsilon
• Host Species: Mouse
• Immunogen: Human infant thymocytes and peripheral blood lymphocytes from a Sézary Syndrome donor
• Purification Method: Affinity Purified
• Quantity: 25 Tests
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Human
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
• Isotype: IgG1 κ

For Research Use Only.