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  6. CD3 Mouse anti-Human, Brilliant Violet 650, Clone: UCHT1, BD

CD3 Mouse anti-Human, Brilliant Violet 650, Clone: UCHT1, BD

Catalog No. BDB563852 Shop All BD Biosciences Products
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Description

Mouse Monoclonal Antibody

The UCHT1 monoclonal antibody specifically binds to the human CD3 ε -chain, a 20-kDa subunit of the CD3/T cell antigen receptor complex. CD3 ε is expressed on 70-80% of normal human peripheral blood lymphocytes and 60-85% of thymocytes. Studies from the HLDA Workshop show that this antibody is mitogenic for CD3 ε -positive cells when used in conjunction with costimulatory agents such as pokeweed mitogen or anti-CD28 antibody. CD3 plays a central role in signal transduction during antigen recognition. The UCHT1 antibody stains both surface and intracellular CD3 ε unlike the other CD3 clone, HIT3a, that stains only extracellular CD3 ε .

The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon™ Brilliant Violet™ family of dyes. This dye is a tandem fluorochrome of BD Horizon™ BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 650-nm. BD Horizon™ BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20-nm filter). Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors. However, the spillover can be corrected through compensation as with any other dye combination.

TRUSTED_SUSTAINABILITY

Specifications

Antigen CD3
Applications Flow Cytometry
Classification Monoclonal
Clone UCHT1
Conjugate Brilliant Violet 650
Description CD3e; CD3E; T3E; TCRE; T-cell surface antigen T3/Leu-4 epsilon
Formulation Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Host Species Mouse
Immunogen Human infant thymocytes and peripheral blood lymphocytes from a Sezary Syndrome donor
Purification Method Affinity Purified
Quantity 100 Tests
Regulatory Status RUO
Primary or Secondary Primary
Target Species Human
Content And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Isotype IgG1 κ
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Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only.

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