CD3ε Hamster anti-Mouse, NA/LE, Unlabeled, Clone: 145-2C11, BD

Hamster Monoclonal Antibody

Supplier:  BD Biosciences 553057

Catalog No. BDB553057

Description

The 145-2C11 antibody reacts with the 25-kDa ε chain of the T-cell receptor-associated CD3 complex, which is expressed on thymocytes, mature T lymphocytes, and NK-T cells. The cytoplasmic domain of CD3e participates in the signal transduction events which activate several cellular biochemical pathways as a result of antigen recognition. Soluble 145-2C11 antibody can activate either unprimed (naive) or primed (memory/preactivated) T cells in vivo or in vitro, in the presence of Fc receptor-bearing accessory cells. In contrast, plate-bound 145-2C11 can activate T cells in the absence of accessory cells. Soluble 145-2C11 antibody has been reported to induce re-directed lysis of Fc receptor-bearing target cells by CTL clones and can also block lysis of specific target cells by antigen-specific CTL's. Under some conditions, T-cell activation by 145-2C11 antibody has been reported to result in apoptotic cell death. The 145-2C11 antibody does not cross-react with rat leukocytes and it has been reported that pre-incubation of thymus cell suspensions at 37°C for 2-4 hours prior to staining enhances the ability of anti-CD3ε and anti-αβ TCR mAbs to detect the T-cell receptor on immature thymocytes. This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

Specifications

• Antigen: CD3 ε
• Classification: Monoclonal
• Concentration: 1.0mg/mL
• Description: CD3; CD3 epsilon; Cd3e; CD3ε; T3e
• Host Species: Hamster
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C

• Applications: Flow Cytometry
• Clone: 145-2C11
• Conjugate: Unconjugated
• Formulation: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered. Endotoxin level is ≤0.01 EU/μg (≤0.001 ng/μg) of protein as determined by the LAL assay.
• Immunogen: H-2Kb specific cytotoxic T lymphocyte clone BM10-37
• Quantity: 0.5 mg
• Research Discipline: Immunology
• Target Species: Bovine, Mouse
• Isotype: IgG1 κ

For Research Use Only.