Learn More
CD3 Mouse anti-Human, Brilliant Violet 510, Clone: UCHT1, BD
Mouse Monoclonal Antibody
Supplier: BD Biosciences 563109
Description
The UCHT1 monoclonal antibody specifically binds to the human CD3ε-chain, a 20-kDa subunit of the CD3/T cell antigen receptor complex. CD3ε is expressed on 70-80% of normal human peripheral blood lymphocytes and 60-85% of thymocytes. Studies from the HLDA Workshop show that this antibody is mitogenic for CD3ε-positive cells when used in conjunction with costimulatory agents such as pokeweed mitogen or anti-CD28 antibody. CD3 plays a central role in signal transduction during antigen recognition. The UCHT1 antibody stains both surface and intracellular CD3ε unlike the other CD3 clone, HIT3a, that stains only extracellular CD3ε.
The antibody was conjugated to BD Horizon™ BV510 which is part of the BD Horizon™ Brilliant Violet™ family of dyes. With an Ex Max of 405-nm and Em Max at 510-nm, BD Horizon™ BV510 can be excited by the violet laser and detected in the BD Horizon™ V500 (525/50-nm) filter set. BD Horizon™ BV510 conjugates are useful for the detection of dim markers off the violet laser.

Specifications
CD3 | |
Monoclonal | |
Brilliant Violet 510 | |
Aqueous buffered solution containing BSA and ≤0.09% sodium azide. | |
Human infant thymocytes and peripheral blood lymphocytes from a Sézary Syndrome donor | |
50 Tests | |
Primary | |
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. |
Flow Cytometry | |
UCHT1 | |
CD3e; CD3E; T3E; TCRE; T-cell surface antigen T3/Leu-4 epsilon | |
Mouse | |
Affinity Purified | |
RUO | |
Human | |
IgG1 κ |
Safety and Handling
Your input is important to us. Please complete this form to provide feedback related to the content on this product.