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  6. CD3 Mouse anti-Human, Brilliant Violet 421, Clone: UCHT1, BD

CD3 Mouse anti-Human, Brilliant Violet 421, Clone: UCHT1, BD

Catalog No. BDB562427 Shop All BD Biosciences Products
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Description

Mouse Monoclonal Antibody

The UCHT1 monoclonal antibody specifically binds to the human CD3ε-chain, a 20-kDa subunit of the CD3/T cell antigen receptor complex. CD3ε is expressed on 70-80% of normal human peripheral blood lymphocytes and 60-85% of thymocytes. Studies from the HLDA Workshop show that this antibody is mitogenic for CD3ε-positive cells when used in conjunction with costimulatory agents such as pokeweed mitogen or anti-CD28 antibody. CD3 plays a central role in signal transduction during antigen recognition. The UCHT1 antibody stains both surface and intracellular CD3ε unlike the other CD3 clone, HIT3a, that stains only extracellular CD3ε.The antibody was conjugated to Brilliant Violet 421 and has been developed in collaboration with Sirigen. With an Ex Max of 407-nm and Em Max at 421-nm, Brilliant Violet 421 can be excited by the violet laser and detected in the standard Pacific Blue filter set (eg, 450/50-nm filter). Brilliant Violet 421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates.

Host Species: Mouse
Clone: UCHT1
Isotype: IgG1, κ
Species Reactivity: Human
Immunogen: Human infant thymocytes and peripheral blood lymphocytes from a Sézary Syndrome donor

Flow Cytometry

TRUSTED_SUSTAINABILITY

Specifications

Antigen CD3
Applications Flow Cytometry
Classification Monoclonal
Clone UCHT1
Conjugate Brilliant Violet 421
Description CD3e; CD3E; T3E; TCRE; T-cell surface antigen T3/Leu-4 epsilon
Formulation Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Host Species Mouse
Immunogen Human infant thymocytes and peripheral blood lymphocytes from a Sézary Syndrome donor
Purification Method Affinity Purified
Quantity 25 Tests
Regulatory Status RUO
Primary or Secondary Primary
Target Species Human
Content And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Isotype IgG1 κ
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For Research Use Only.

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