CD3 Molecular Complex Rat anti-Mouse, Brilliant Violet 421, Clone: 17A2, BD

Catalog No.	Quantity
BDB564008	50 μg

Catalog No. BDB564008 Supplier BD Biosciences Supplier No. 564008

Description

Rat Monoclonal Antibody

The 17A2 monoclonal antibody specifically binds to the T-cell receptor-associated CD3 complex that is expressed on many thymocytes and mature T lymphocytes. Plate-bound 17A2 antibody has been reported to induce IL-2 production by cultured T cells in the absence of accessory cells. The binding of the 17A2 antibody to T cells can be blocked by the anti-CD3e mAb 145-2C11. This suggests that the 17A2 antibody recognizes an epitope of the CD3 epsilon chain. In vivo treatment with 17A2 antibody has been reported to partially deplete T lymphocytes and temporarily down-modulates CD3 expression on T cells.

The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon™ Brilliant Violet™ family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon™ BV421can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon™ BV421conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue™ conjugates.

Specifications

• Antigen: CD3 Molecular Complex
• Applications: Flow Cytometry
• Classification: Monoclonal
• Clone: 17A2
• Concentration: 0.2mg/mL
• Conjugate: Brilliant Violet 421
• Description: CD3; CD3 epsilon; Cd3e; CD3ε; T3e
• Formulation: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
• Host Species: Rat
• Immunogen: γ δ TCR-positive T-T hybridoma D1
• Purification Method: Affinity Purified
• Quantity: 50 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Mouse
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
• Isotype: IgG2b κ