CD3 Molecular Complex Rat anti-Mouse, V450, Clone: 17A2, BD

Catalog No.	Quantity
BDB561389	50 μg

Catalog No. BDB561389 Supplier BD Biosciences Supplier No. 561389

Description

Rat Monoclonal Antibody

The 17A2 monoclonal antibody specifically binds to the T-cell receptor-associated CD3 complex that is expressed on many thymocytes and mature T lymphocytes. Plate-bound 17A2 antibody has been reported to induce IL-2 production by cultured T cells in the absence of accessory cells. The binding of the 17A2 antibody to T cells can be blocked by the anti-CD3e mAb 145-2C11. This suggests that the 17A2 antibody recognizes an epitope of the CD3 epsilon chain. In vivo treatment with 17A2 antibody has been reported to partially deplete T lymphocytes and temporarily down-modulates CD3 expression on T cells.

The antibody is conjugated to BD Horizon™ V450, which has been developed for use in multicolor flow cytometry experiments and is available exclusively from BD Biosciences. It is excited by the Violet laser Ex max of 406nm and has an Em Max at 450 nm. Conjugates with BD Horizon™ V450 can be used in place of Pacific Blue™ conjugates.

Specifications

• Antigen: CD3 Molecular Complex
• Applications: Flow Cytometry
• Classification: Monoclonal
• Clone: 17A2
• Concentration: 0.2mg/mL
• Conjugate: Violet Fluor 450 (V450)
• Formulation: Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
• Host Species: Rat
• Immunogen: γδ TCR-positive T-T hybridoma D1
• Purification Method: Affinity Purified
• Quantity: 50 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Mouse
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
• Isotype: IgG2b κ