CD3ε Hamster anti-Mouse, PE, Clone: 145-2C11, BD

Hamster Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
BDB553063	BD Biosciences 553063	0.1 mg
BDB553064	BD Biosciences 553064	0.2 mg

Description

The 145-2C11 antibody reacts with the 25-kDa ε chain of the T-cell receptor-associated CD3 complex, which is expressed on thymocytes, mature T lymphocytes, and NK-T cells. The cytoplasmic domain of CD3e participates in the signal transduction events which activate several cellular biochemical pathways as a result of antigen recognition. Soluble 145-2C11 antibody can activate either unprimed (naive) or primed (memory/preactivated) T cells in vivo or in vitro, in the presence of Fc receptor-bearing accessory cells. In contrast, plate-bound 145-2C11 can activate T cells in the absence of accessory cells. Soluble 145-2C11 antibody has been reported to induce re-directed lysis of Fc receptor-bearing target cells by CTL clones and can also block lysis of specific target cells by antigen-specific CTL's. Under some conditions, T-cell activation by 145-2C11 antibody has been reported to result in apoptotic cell death. The 145-2C11 antibody does not cross-react with rat leukocytes and it has been reported that pre-incubation of thymus cell suspensions at 37°C for 2-4 hours prior to staining enhances the ability of anti-CD3ε and anti-αβ TCR mAbs to detect the T-cell receptor on immature thymocytes. This antibody is routinely tested by flow cytometric analysis and is compatible with intracellular staining protocols using the BD Cytofix/Cytoperm™ Kit (Cat. No. 554714). Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

Host Species: Hamster
Clone: 145-2C11
Isotype: IgG1 κ
Species Reactivity: Mouse
Immunogen: H-2Kb specific cytotoxic T lymphocyte clone BM10-37

Flow Cytometry, Intracellular Staining

Specifications

• Antigen: CD3 ε
• Concentration: 0.2mg/mL
• Classification: Monoclonal
• Host Species: Hamster
• Research Discipline: Immunology
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Isotype: IgG1 κ
• Purification Method: Affinity Purified
• Description: CD3; CD3 epsilon; Cd3e; CD3ε; T3e

• Clone: 145-2C11
• Applications: Flow Cytometry
• Conjugate: PE
• Regulatory Status: RUO
• Target Species: Mouse
• Immunogen: H-2Kb specific cytotoxic T lymphocyte clone BM10-37
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

For Research Use Only.