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Description
The 1F4 antibody reacts with the T-cell receptor-associated CD3 cell-surface antigen found on thymocytes, peripheral T lymphocytes, and dendritic epidermal T cells. In vitro, immobilized 1F4 mAb induces T-cell proliferation, and soluble mAb synergizes with PMA for T-cell activation. Pre-incubation of splenocytes with mAb 1F4 blocks staining with mAb G4.18 (Cat. No. 559974/554830).
This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
Specifications
Specifications
| Antigen | CD3 |
| Applications | Flow Cytometry |
| Classification | Monoclonal |
| Clone | 1F4 |
| Concentration | 0.2mg/mL |
| Conjugate | PE |
| Formulation | Aqueous buffered solution containing ≤0.09% sodium azide. |
| Host Species | Mouse |
| Immunogen | F344 rat splenic T cells stimulated with PMA plus A23187 |
| Purification Method | Affinity Purified |
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Safety and Handling
For Research Use Only.
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