CD31 Rat anti-Mouse, Clone: ER-MP12, Invitrogen
Rat Monoclonal Antibody
Manufacturer: Invitrogen MA140074
MA1-40074 detects CD31 from mouse samples. MA1-40074 reacts with an antigen present on cell subpopulations of adult mouse bone marrow, including pluripotent hematopoietic stem cells. MA1-40074 reacts with the major population of colony forming unit macrophage (CFU-M) precursor cells, with subpopulation of pre CFU-M and monoblasts, and with precursor cells of granulocytes. MA1-40074 has been successfully used in ELISA, immunohistochemistry (paraffin and frozen), immunoprecipitation, and flow cytometry applications. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting, working dilution is 1:50. The MA1-40074 immunogen is mouse PECAM-1/CD31. Suggested positive control: Macrophage cell line RMB-1.The mouse hematopoietic progenitor cell antigen is Platelet-Endothelial Cell Adhesion Molecule (PECAM)-1 (CD31) of 113 kDa. PECAM-1 is a member of the immunoglobulin superfamily.This heavily glycosylated protein is found in the entire vascular endothelium of adult mice, making it a commonly used endothelial marker, and functions in mediating cellular adhesion by heterophilic and homophilic mechanisms. The type I transmembrane protein is composed of an extracellular region of six Ig-like homology domains, a 19-residue transmembrane domain, and a 118 residue cytoplasmic tail and is differentially glycosylated involving N-linked and Olinked glycosylation sites. The signalling of PECAM-1 is based on 2 immuno receptor tyrosine inhibitory motifs (ITIMs) in its cytoplasmic domain. These ITIM domains can serve as docking sites for signalling molecules such as protein tyrosine phosphatases, and ligation of PECAM-1 can induce phosphorylation of tyrosine- and serine/threonineresidues of these intracellular regions eventually leading to activation of signalling pathways. The biological characteristics of PECAM-1 in these pathways and cellular adhesion have been mapped to specific regions of the PECAM-1 molecule. PECAM-1 is deteted within the lyphopoietic islands in the spleen of newborn (day 12) and the bone marrow of adult mice. Capillary endothelial cells of adult mice also express PECAM-1. Furthermore, it is expressed on most cells of the hematopoietic lineage including platelets, monocytes, neutrophils, and lymphocyte subsets. Besides adhesive properties PECAM-1 is an efficient signalling molecule. The protein is involved in vascular biology including angiogenesis, platelet function, and thrombosis, mechanosensing of endothelial cells, and regulation of multiple stages of leukocyte migration through venular walls.
|PBS with 0.1% BSA and 0.02% sodium azide|
|CD31 antigen, CD31/EndoCAM, GPIIA′, PECA1, PECAM-1, CD31/EndoCAM, adhesion molecule, endoCAM, platelet endothelial cell adhesion molecule|
|ELISA, Flow Cytometry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation|
|Murine macrophage precursor hybrid W1C3cell hybrid|
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