CD33 Mouse anti-Human, BB515, Clone: WM53, BD

Mouse Monoclonal Antibody

Supplier:  BD Biosciences 564588

Catalog No. BDB564588

Description

The WM53 monoclonal antibody specifically binds to human CD33. CD33 is a 67 kDa type I transmembrane glycoprotein. It is expressed on monocytes, activated T cells, myeloid progenitors, mast cells and weakly on polymorphonuclear cells. CD33 is absent on normal platelets, lymphocytes, erythrocytes, and hematopoietic stem cells. Reports indicate that this glycoprotein can function as a sialic acid-dependent cell adhesion molecule and this function can be modulated by endogenous sialoglycoconjugates when CD33 is expressed on the membrane.

The antibody was conjugated to BD Horizon BB515 which was developed exclusively by BD Biosciences. With an excitation max of 490nm and an emission max of 515nm, BD Horizon BB515 can be excited by the 488nm laser and detected in a standard FITC set (eg, 530/30-nm filter). This dye provides a much brighter alternative to FITC with less spillover into the PE detector.

Specifications

• Antigen: CD33
• Classification: Monoclonal
• Conjugate: Brilliant Blue 515 (BB515)
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide
• Immunogen: Acute Myeloid Leukemia Blasts
• Quantity: 100 Tests
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

• Applications: Flow Cytometry
• Clone: WM53
• Description: Siglec-3; SIGLEC3; Sialic acid-binding Ig-like lectin 3; p67; gp67; My9
• Host Species: Mouse
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Target Species: Human
• Isotype: IgG1 κ

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov