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CD33 Mouse anti-Human, V450, Clone: WM53 (also known as WM-53), BD

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Catalog No. BDB561157 Supplier BD Biosciences Supplier No. 561157
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Description

Mouse Monoclonal Antibody

The WM53 monoclonal antibody specifically binds to human CD33. CD33 is a 67 kDa type I transmembrane glycoprotein. It is expressed on monocytes, activated T cells, myeloid progenitors, mast cells and weakly on polymorphonuclear cells. CD33 is absent on normal platelets, lymphocytes, erythrocytes, and hematopoietic stem cells. Reports indicate that this glycoprotein can function as a sialic acid-dependent cell adhesion molecule and this function can be modulated by endogenous sialoglycoconjugates when CD33 is expressed on the membrane.

The antibody is conjugated to BD Horizon™ V450, which has been developed for use in multicolor flow cytometry experiments and is available exclusively from BD Biosciences. It is excited by the Violet laser Ex max of 406nm and has an Em Max at 450 nm. Conjugates with BD Horizon™ V450 can be used in place of Pacific Blue™ conjugates.

TRUSTED_SUSTAINABILITY

Specifications

Antigen CD33
Applications Flow Cytometry
Classification Monoclonal
Clone WM53 (also known as WM-53)
Conjugate Violet Fluor 450 (V450)
Description Siglec-3; SIGLEC3; Sialic acid-binding Ig-like lectin 3; p67; gp67; My9
Formulation Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
Host Species Mouse
Immunogen Acute Myeloid Leukemia Blasts
Purification Method Affinity Purified
Quantity 50 Tests
Regulatory Status RUO
Primary or Secondary Primary
Target Species Human
Content And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Isotype IgG1 κ
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