CD335 Mouse anti-Human, BB515, Clone: αR1, BD
Mouse Monoclonal Antibody
Manufacturer: BD Biosciences 564594
The αR1 monoclonal antibody specifically binds to the human platelet derived growth factor (PDGF) receptor α (PDGFR α ), also known as CD140a. CD140a is a 170 kDa single transmembrane glycoprotein expressed on fibroblasts, smooth muscle cells, glial cells a nd chondrocytes. PDGF receptors α and β are single glycoproteins with intracellular tyrosine kinase domains. They are structurally similar to the M-CSF receptor and CD117 (c-kit). Their ligand, PDGF, is a mitogen for connective tissue cells and glial cells. PDGF plays a role in wound healing and it also acts as a chemoattractant for fibroblasts, smooth muscle cells, glial cells, monocytes and neutrophils. Functional PDGF is secreted in disulfide linked, homodimeric or heterodimeric forms comprised of A or B chains (PDGFAA, PDGF-BB or PDGF-AB). Binding of divalent PDGF induces receptor dimerization with three possible form s: αα, αβ, ββ. The PD GFR α subunit binds both PDGF A and B chains, whereas the PDGFR β subunit binds only PDGF B chains. Although both receptor subunits can stimulate mitogenic responses, only the β subunit can induce chemotaxis. The αR1 antibody is specific for PDGFR α and does not crossreact with PDGFR β . It immunoprecipitates human, monkey, rabbit, pig, dog and cat PDGFR α . It does not recognize hamster, rat or mouse PDGFR α .
The antibody was conjugated to BD Horizon BB515 which was developed exclusively by BD Biosciences. With an excitation max of 490nm and an emission max of 515nm, BD Horizon BB515 can be excited by the 488nm laser and detected in a standard FITC set (eg, 530/30-nm filter). This dye provides a much brighter alternative to FITC with less spillover into the PE detector.
|PDGF Receptor α; PDGFRA; PDGFRα; PDGF-R-alpha; PDGFR2; PGFRA; RHEPDGFRA|
|Human PDGFRα Transfected Cell Line|
|Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.|
|Aqueous buffered solution containing ≤0.09% sodium azide|
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