CD39 Mouse anti-Human, Brilliant Violet 650, Clone: TU66 (also known as Tü 66, Tü66), BD

Mouse Monoclonal Antibody

Supplier:  BD Biosciences 563681

Catalog No. BDB563681

Description

The TU66 monoclonal antibody reacts with human CD39 also known as ectonucleoside triphosphate diphosphohydrolase 1 (ENTPD1) an ectoenzyme that degrades ATP to AMP. It is a member of the family of ectonucleoside triphosphate dihydrolases (E-NTPDases) known to be involved in regulation of extracellular nucleotide catabolism, controlling the extracellular nucleoside triphosphate pool (NTP). CD39 is expressed on a subset of T cells, B cells and dendritic cells with weak staining of monocytes and granulocytes. Recently, CD39 has been found to be expressed primarly by immune-suppressive Foxp3(+) regulatory T (Treg) cells in both human and mice. In humans, CD39 is restricted to a subset of Foxp3+ regulatory effector/memory-like T cells. In mice, the enzyme is present on most if not all CD4+CD25+ cells and CD39 expression is driven by Foxp3. It is thought that CD39 allows Treg cells to enter inflamed areas where high levels of ATP are present.

The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon™ Brilliant Violet™ family of dyes. This dye is a tandem fluorochrome of BD Horizon™ BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 650-nm. BD Horizon™ BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20-nm filter). Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors. However, the spillover can be corrected through compensation as with any other dye combination.

Specifications

• Antigen: CD39
• Classification: Monoclonal
• Conjugate: Brilliant Violet 650
• Host Species: Mouse
• Quantity: 100 Tests
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

• Applications: Flow Cytometry
• Clone: TU66 (also known as Tü 66, Tü66)
• Description: ENTPD1; NTPDase-1; Ecto-ATPase 1; Ecto-ATPDase 1
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Target Species: Human
• Isotype: IgG2b κ

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov