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Description
This antibody has been reported to react with the 25-kDa ε chain of the T-cell receptor-associated CD3 complex expressed on thymocytes, mature T lymphocytes, and NK-T cells on many mouse stains. Plate-bound and soluble hamster anti-mouse CD3e (clone 500A2) antibody has been reported to activate unprimed T cells in vitro. Activation of a mouse T-cell clone by soluble hamster anti-mouse CD3e (clone 500A2) can be blocked by Fab fragments of anti-CD4 antibody GK1.5, suggesting that the hamster anti-mouse CD3e (clone 500A2) antibody may bind an epitope of CD3e close to a site at which CD4 associates with the T-cell receptor. This hamster mAb to a mouse leukocyte antigen has been reported not to cross-react with rat leukocytes.
The antibody is conjugated to BD Horizon™ V450, which has been developed for use in multicolor flow cytometry experiments and is available exclusively from BD Biosciences. It is excited by the 450 nm. Conjugates with BD Horizon™ V450 can be used in place of Pacific Blue™ conjugates.
Specifications
Specifications
| Antigen | CD3e |
| Applications | Flow Cytometry |
| Classification | Monoclonal |
| Clone | 500A2 |
| Concentration | 0.2mg/mL |
| Conjugate | Violet Fluor 450 (V450) |
| Description | CD3ε chain |
| Formulation | Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide. |
| Host Species | Hamster |
| Immunogen | Mouse T-cell receptor |
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For Research Use Only.
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