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CD3e Hamster anti-Mouse, V450, Clone: 500A2, BD

Catalog No. BDB560804 Shop All BD Biosciences Products
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0.1 mg
25 μg
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BDB560804 25 μg
BDB560801 0.1 mg
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Catalog No. BDB560804 Supplier BD Biosciences Supplier No. 560804
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Description

Hamster Monoclonal Antibody

This antibody has been reported to react with the 25-kDa ε chain of the T-cell receptor-associated CD3 complex expressed on thymocytes, mature T lymphocytes, and NK-T cells on many mouse stains. Plate-bound and soluble hamster anti-mouse CD3e (clone 500A2) antibody has been reported to activate unprimed T cells in vitro. Activation of a mouse T-cell clone by soluble hamster anti-mouse CD3e (clone 500A2) can be blocked by Fab fragments of anti-CD4 antibody GK1.5, suggesting that the hamster anti-mouse CD3e (clone 500A2) antibody may bind an epitope of CD3e close to a site at which CD4 associates with the T-cell receptor. This hamster mAb to a mouse leukocyte antigen has been reported not to cross-react with rat leukocytes.

The antibody is conjugated to BD Horizon™ V450, which has been developed for use in multicolor flow cytometry experiments and is available exclusively from BD Biosciences. It is excited by the 450 nm. Conjugates with BD Horizon™ V450 can be used in place of Pacific Blue™ conjugates.

TRUSTED_SUSTAINABILITY

Specifications

Antigen CD3e
Applications Flow Cytometry
Classification Monoclonal
Clone 500A2
Concentration 0.2mg/mL
Conjugate Violet Fluor 450 (V450)
Description CD3ε chain
Formulation Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
Host Species Hamster
Immunogen Mouse T-cell receptor
Purification Method Affinity Purified
Quantity 25 μg
Regulatory Status RUO
Primary or Secondary Primary
Target Species Mouse
Content And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Isotype IgG2 κ
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For Research Use Only.

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