MilliporeSigma™ anti-CD4 APC, Clone: RM4-5,
Rat Monoclonal Antibody
Manufacturer: MilliporeSigma™ MABF1566
Specifically detects CD4 Clone: RM4-5 in Mouse samples, and it is validated for Flow CytometryThe RM4-5 antibody reacts with mouse CD4, a 55kDa protein which acts as a co-receptor for the T cell receptor (TCR) in its interaction with MHC Class II molecules on antigen-presenting cells. The extracellular domain of CD4 binds to the beta-2 domain of MHC Class II, while its cytoplasmic tail provides a binding site for the tyrosine kinase lck, facilitating the signaling cascade that initiates T cell activation. CD4 is typically expressed on thymocytes, certain mature T cell populations such as Th17 and T regulatory (Treg) cells, as well as on dendritic cells.The RM4-5 antibody is widely used as a phenotypic marker for CD4 expression. If used together, the RM4-5 antibody and an alternative antibody, Anti-Mouse CD4 clone GK1.5, will 'compete' for binding, i.e. RM4-5 antibody is able to block GK1.5 antibody binding to cells. In contrast, RM4-5 antibody does not block the binding of Anti-Mouse CD4 clone RM4-4 to cells.
|Purified Rat monoclonal containing 10mM NaH2PO4, 150mM NaCl, 0.09% NaN3, 0.1% gelatin, pH 7.2.|
|Purified by size-exclusion chromatography|
|2°C to 8°C undiluted for one year from date of shipment protected from light; Do not freeze|
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