CD4 Mouse anti-Human, Brilliant Violet 650, Clone: L200, BD
Mouse Monoclonal Antibody
Manufacturer: BD Biosciences 563737
The L200 monoclonal antibody specifically binds to the human form of the 56 kDa transmembrane glycoprotein, CD4, present on the T-helper/inducer subset of normal human donor peripheral blood lymphocytes. The L200 antibody also crossreacts with a subset of CD3-positive peripheral blood lymphocytes, but not monocytes, of both Rhesus and Cynomolgus Macaque monkeys. Crossreactivity on both lymphocytes and monocytes (weak) from Baboons is also observed. The distribution on lymphocytes is similar for both human and monkey cells, with the majority of CD4-positive lymphocytes being CD8-negative and lacking reactivity with antibodies to B- or NK-cell markers.
The antibody was conjugated to BD Horizon BV650 which is part of the BD Horizon Brilliant Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 650-nm. BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20-nm filter). Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor 700 detectors. However, the spillover can be corrected through compensation as with any other dye combination.
|L3T4 ; T-cell surface antigen T4/Leu-3; W3/25 ; CD4 antigen (p55)|
|Human HPB-ALL Cell Line|
|Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.|
|Brilliant Violet 650|
|Aqueous buffered solution containing BSA and ≤0.09% sodium azide.|
|Baboon, Cynomolgus, Rhesus|
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