CD4 Rat anti-Mouse, NA/LE, Unlabeled, Clone: GK1.5, BD

Rat Monoclonal Antibody

Supplier:  BD Biosciences 553726

Catalog No. BDB553726

Description

The GK1.5 clone has been reported to react with the CD4 (L3T4) differentiation antigen expressed on most thymocytes, a subpopulation of mature T lymphocytes (i.e., MHC class II-restricted T cells, including most T helper cells), and a subset of NK-T cells. In addition, CD4 has also been reported to be detectable on pluripotent hematopoietic stem cells, bone marrow myeliod and B-lymphocyte precursors, intrathymic lymphoid precursors, and a subset of splenic dendritic cells. CD4 has also been reported to be expressed on the plasma membrane of mouse egg cells and is involved in adhesion of the egg to MHC class II-bearing sperm. CD4 is an antigen coreceptor on the T-cell surface which interacts with MHC class II molecules on antigen-presenting cells. It participates in T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase lck. GK1.5 mAb reportedly blocks binding of the RM4-5 (Cat. No. 553046/553047) and H129.19 (Cat. No. 553650/553651), but not RM4-4 (Cat. No. 553055) antibodies. This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

Specifications

• Antigen: CD4
• Classification: Monoclonal
• Concentration: 1.0mg/mL
• Description: Cd4; CD4 antigen; L3T4; Ly-4; T-cell surface antigen T4/Leu-3
• Host Species: Rat
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C

• Applications: Flow Cytometry
• Clone: GK1.5
• Conjugate: Unconjugated
• Formulation: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered. Endotoxin level is ≤0.01 EU/μg (≤0.001 ng/μg) of protein as determined by the LAL assay.
• Immunogen: Mouse CTL clone V4
• Quantity: 0.5 mg
• Research Discipline: Immunology
• Target Species: Mouse
• Isotype: IgG2b κ

For Research Use Only.