MilliporeSigma™ anti-CD4 FITC, Clone: RM4-5,

Rat Monoclonal Antibody

Supplier:  MilliporeSigma™ MABF1568

Catalog No. MABF1568MI

Description

Specifically detects CD4 Clone: RM4-5 in Mouse samples, and it is validated for Flow Cytometry

The RM4-5 antibody reacts with mouse CD4, a 55kDa protein which acts as a co-receptor for the T cell receptor (TCR) in its interaction with MHC Class II molecules on antigen-presenting cells. The extracellular domain of CD4 binds to the beta2-domain of MHC Class II, while its cytoplasmic tail provides a binding site for the tyrosine kinase lck, facilitating the signaling cascade that initiates T cell activation. CD4 is typically expressed on thymocytes, certain mature T cell populations such as Th17 and T regulatory (Treg) cells, as well as on dendritic cells.The RM4-5 antibody is widely used as a phenotypic marker for CD4 expression. If used together, the RM4-5 antibody and an alternative antibody, Anti-Mouse CD4 clone GK1.5, will 'compete' for binding, i.e. RM4-5 antibody is able to block GK1.5 antibody binding to cells. In contrast, RM4-5 antibody does not block the binding of Anti-Mouse CD4 clone RM4-4 to cells.

Specifications

• Antigen: CD4
• Classification: Monoclonal
• Conjugate: FITC
• Gene Accession No.: P06332
• Host Species: Rat
• Purification Method: Purified by size-exclusion chromatography
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Content And Storage: 2°C to 8°C undiluted for one year from date of shipment protected from light; Do not freeze
• Isotype: IgG2a κ

• Applications: Flow Cytometry
• Clone: RM4-5
• Formulation: Purified Rat monoclonal containing 10mM NaH2PO4, 150mM NaCl, 0.09% NaN3, 0.1% gelatin, pH 7.2.
• Gene Symbols: Cd4
• Immunogen: Proprietary immunogen
• Quantity: 100 μg
• Research Discipline: Inflammation, Immunology
• Target Species: Mouse
• Form: Purified

For Research Use Only