CD4 Rat anti-Mouse, PE, Clone: H129.19, BD

Rat Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
BDB553652	BD Biosciences 553652	0.1 mg
BDB553653	BD Biosciences 553653	0.2 mg

Description

The H129.19 antibody reacts with the CD4 (L3T4) differentiation antigen expressed on thymocytes, a subpopulation of mature T lymphocytes (i.e., MHC class II-restricted T cells, including most T helper cells), and a subset of NK-T cells of all mouse strains tested. CD4 has also been detected on pluirpotent hematopoietic stem cells, bone marrow myeloid precursors, intrathymic lymphoid precursors, and a subset of splenic dendritic cells. CD4 is expressed on the plasma membrane of mouse egg cells and is involved in adhesion of the egg to MHC class II-bearing sperm. CD4 is an antigen coreceptor on the T-cell surface which interacts with MHC class II molecules on antigen-presenting cells. It participates in T-cell activation through its association with the T-cell receptor complex and protein tyrosine lck. H129.19 mAb blocks binding of the anti-mouse CD4 mAbs Gk1.5 (Cat. No. 557307/553729) and RM4-5 (Cat. No. 553046/553047), but not RM4-4 (Cat. No. 553055) antibody. mAb H129.19 inhibits various responses of T helper cells to antigenic or mitogenic stimuli. This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

Host Species: Rat
Clone: H129.19
Isotype: IgG2a κ
Species Reactivity: Mouse
Immunogen: A.TH mouse CTL clone A15.1.17

Flow Cytometry

Specifications

• Antigen: CD4
• Concentration: 0.2mg/mL
• Classification: Monoclonal
• Host Species: Rat
• Research Discipline: Immunology
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Isotype: IgG2a κ
• Purification Method: Affinity Purified
• Description: L3T4

• Clone: H129.19
• Applications: Flow Cytometry
• Conjugate: PE
• Regulatory Status: RUO
• Target Species: Mouse
• Immunogen: A.TH mouse CTL clone A15.1.17
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

For Research Use Only.