MilliporeSigma™ anti-CD4 Red Fluor 710, Clone: GK1.5,

Rat Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
MABF1576MI	MilliporeSigma™ MABF1576	100 μg

Description

Specifically detects CD4 Clone: GK1.5 in Mouse samples, and it is validated for Flow Cytometry

The GK1.5 antibody reacts with mouse CD4, a 55kDa protein which acts as a co-receptor for the T cell receptor (TCR) in its interaction with MHC Class II molecules on antigen-presenting cells. The extracellular domain of CD4 binds to the beta-2 domain of MHC Class II, while its cytoplasmic tail provides a binding site for the tyrosine kinase lck, facilitating the signaling cascade that initiates T cell activation. CD4 is typically expressed on thymocytes, certain mature T cell populations such as Th17 and T regulatory (Treg) cells, as well as on dendritic cells.The GK1.5 antibody is widely used as a phenotypic marker for CD4 expression. If used together, the GK1.5 antibody and an alternative antibody, Anti-Mouse CD4 clone RM4-5, will 'compete' for binding, i.e. RM4-5 is able to block GK1.5 binding to cells. In contrast, the Anti-Mouse CD4 clone RM4-4 does not block binding of the GK1.5 antibody to cells (Arora S et al. 2006. Infect. Immun. 74: 4339-4348). The GK1.5 antibody is also reported to be cross-reactive with Syrian hamster CD4.

Specifications

• Antigen: CD4
• Applications: Flow Cytometry
• Conjugate: Red Fluor 710
• Host Species: Rat
• Research Discipline: Inflammation, Immunology
• Formulation: Purified Rat monoclonal containing 10mM NaH2PO4, 150mM NaCl, 0.09% NaN3, 0.1% gelatin, pH 7.2.
• Gene Symbols: Cd4
• Primary or Secondary: Primary
• Content And Storage: 2°C to 8°C undiluted for one year from date of shipment protected from light; Do not freeze

• Clone: GK1.5
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Mouse
• Gene Accession No.: P06332
• Immunogen: Proprietary immunogen
• Purification Method: Purified by size-exclusion chromatography

For Research Use Only