CD4 Mouse anti-Human, V500, Clone: L200, BD
Mouse Monoclonal Antibody
Manufacturer: BD Biosciences 561488
The L200 monoclonal antibody specifically binds to the human form of the 56 kDa transmembrane glycoprotein, CD4, present on the T-helper/inducer subset of normal human donor peripheral blood lymphocytes. The L200 antibody also crossreacts with a subset of CD3-positive peripheral blood lymphocytes, but not monocytes, of both Rhesus and Cynomolgus Macaque monkeys. Crossreactivity on both lymphocytes and monocytes (weak) from Baboons is also observed. The distribution on lymphocytes is similar for both human and monkey cells, with the majority of CD4-positive lymphocytes being CD8-negative and lacking reactivity with antibodies to B- or NK-cell markers.
The antibody is conjugated to BD Horizon™ V500, which has been developed for use in multicolor flow cytometry experiments and is
available exclusively from BD Biosciences. It is excited by the Violet laser with an Ex max of 415nm and Em Max at 500 nm. BD Horizon V500 conjugates emit at a similar wavelength to Amcyan yet exhibit reduced spillover into the FITC channel. For more information on BD Horizon V500, visit bdbiosciences.com/colors.
|Aqueous buffered solution containing protein stabilizer, glycerol and ≤0.09% sodium azide.|
|Baboon, Cynomolgus, Rhesus Monkey|
|Human HPB-ALL Cell Line|
|Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.|
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