CD40 Mouse anti-Human, V450, Clone: 5C3, BD

Catalog No.	Quantity
BDB561219	50 Tests

Catalog No. BDB561219 Supplier BD Biosciences Supplier No. 561219

Description

Mouse Monoclonal Antibody

This 5C3 monoclonal antibody specifically binds to CD40, a 45-48 kDa type I integral membrane glycoprotein. CD40 is expressed on B lymphocytes, but is not expressed on terminally differentiated B cells. CD40 is also expressed by endothelial cells, basal epithelial cells and some epithelial cell carcinomas, follicular dendritic cells, macrophages, fibroblasts, keratinocytes, and CD34+ hematopoietic progenitor cells. This antibody is useful for studying the roles played by CD40 in B-cell growth, proliferation, and differentiation including immunoglobulin isotype switching. Anti-CD40 antibodies have been reported to stimulate B-cell proliferation when costimulated with anti-µ, anti-CD20 antibodies or with phorbol esters. 5C3 is capable of inducing B-cell proliferation when presented with IL-4.

The antibody is conjugated to BD Horizon V450, which has been developed for use in multicolor flow cytometry experiments and is available exclusively from BD Biosciences. It is excited by the Violet laser Ex max of 406nm and has an Em Max at 450 nm. Conjugates with BD Horizon V450 can be used in place of Pacific Blue™ conjugates.

Specifications

• Antigen: CD40
• Applications: Flow Cytometry
• Classification: Monoclonal
• Clone: 5C3
• Conjugate: Violet Fluor 450 (V450)
• Description: TNFRSF5; TNF receptor superfamily member 5; CD40L receptor; Bp50; p50
• Formulation: Aqueous buffered solution containing protein stabilizer, glycerol and ≤0.09% sodium azide.
• Host Species: Mouse
• Purification Method: Affinity Purified
• Quantity: 50 Tests
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Human
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
• Isotype: IgG1 κ