CD44 Mouse anti-Human, BUV737, Clone: G44-26, BD
Mouse Monoclonal Antibody
Manufacturer: BD Biosciences 564941
The G44-26 monoclonal antibody specifically binds to the 80-95 kDa, glycosylated type I transmembrane protein, also known as phagocytic glycoprotein-1 (Pgp-1). CD44 is the receptor for hyaluronic acid. CD44 is expressed on leucocytes, erythrocytes, epithelial cells and weakly on platelets. CD44 is also called extracellular matrix receptor type III and has functional roles in cell migration, lymphocyte homing and adhesion during hematopoiesis and lymphocyte activation. This antibody is suitable for staining formalin-fixed, paraffin-embedded tissue sections using citrate buffer pretreatment. This antibody recognizes the epitope 1 of CD44 antigen according to the HLDA workshop studies.
The antibody was conjugated to BD Horizon BUV737 which is part of the BD Horizon Brilliant Ultraviolet family of dyes. This dye is a tandem fluorochrome of BD Horizon BUV395 with an Ex Max of 348-nm and an acceptor dye with an Em Max at 737-nm. BD Horizon Brilliant BUV737 can be excited by the ultraviolet laser (355 nm) and detected with a 740/35 filter. Due to the excitation of the acceptor dye by other laser lines, there may be significant spillover into channels detecting Alexa Fluor 700-like dyes (e.g., 712/20-nm filter).
|Phagocytic glycoprotein 1; Pgp-1; H-CAM; Hermes; ECMR III; HUTCH-1|
|Brilliant Ultraviolet 737|
|Aqueous buffered solution containing ≤0.09% sodium azide|
|Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.|
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