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CD45.2 Mouse anti-Mouse, Brilliant Violet 786, Clone: 104, BD
Mouse Monoclonal Antibody
Supplier: BD Biosciences 563686
Description
The 104 clone has been reported to react with CD45 (Leukocyte Common Antigen) on all leukocytes of most mouse strains (eg, A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C57BR, C57L, C58, DBA/1, DBA/2, NZB, SWR, 129). This alloantigen was originally named Ly-5.1, and this was the designation at the time that the antibody was characterized. The designation was later changed from Ly-5.1 to Ly-5.2 to conform with the convention that the .2 alloantigen designations be assigned to the C57BL/6 strain. mAb 104 has been reported not to react with leukocytes of the mouse strains expressing the CD45.1 alloantigen (eg, RIII, SJL/J, STS/A, and DA). CD45 is a member of the Protein Tyrosine Phosphatase (PTP) family: its intracellular (COOH-terminal) region contains two PTP catalytic domains, and the extracellular region is highly variable due to alternative splicing of exons 4, 5, and 6 (designated A, B, and C, respectively), plus differing levels of glycosylation. The CD45 isoforms detected in the mouse are cell type-, maturation-, and activation state-specific. The CD45 isoforms play complex roles in T-cell and B-cell antigen receptor signal transduction. The 104 antibody has been reported to inhibit some responses of B cells, from mice expressing the CD45.2 alloantigen, to certain antigens and LPS. In addition, reduction of serum IgG levels and amelioration of autoimmune renal pathology were reported in mAb 104-treated systemic lupus erythematosus-prone mice.
The antibody was conjugated to BD Horizon™ BV786 which is part of the BD Horizon™ Brilliant Violet™ family of dyes. This dye is a tandem fluorochrome of BD Horizon™ BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 786-nm. BD Horizon™ BV786 can be excited by the violet laser and detected in a filter used to detect Cy7™-like dyes (eg, 780/60-nm filter).
Specifications
CD45.2 | |
Monoclonal | |
0.2mg/mL | |
Ly-5.2; T200; LCA; Leukocyte common antigen; Ptprc | |
B10.S mouse thymocytes and splenocytes | |
50 μg | |
Primary | |
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. |
Flow Cytometry | |
104 | |
Brilliant Violet 786 | |
Mouse | |
Affinity Purified | |
RUO | |
Mouse | |
IgG2a κ |
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