CD45 Mouse, Brilliant Violet 786, Clone: D058-1283, BD
Mouse Monoclonal Antibody
Manufacturer: BD Biosciences 563861
D058-1283 is a CD45 monoclonal antibody specific for non-human primate leucocytes, developed using Rhesus peripheral whole blood as immunogen. It does not cross react with human leucocytes. This antibody reacts with Baboon, Rhesus and Cynomolgus Macaque leucocytes in a similar pattern as seen with CD45 binding to the Leukocyte Common Antigen on human cells. Immunophenotypic analysis shows that D058-1283 binds to lymphocytes, monocytes and granulocytes of non-human primate blood samples. This antibody is able to block the binding of monoclonal antibody TÜ116; a reported anti-human CD45 antibody that cross-reacts with nonhuman primate leucocytes. In Western blot analysis, the D058-1283 antibody identifies a band of approximately 180-200 kDa molecular weight .
The antibody was conjugated to BD Horizon BV786 which is part of the BD Horizon Brilliant Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 786-nm. BD Horizon BV786 can be excited by the violet laser and detected in a filter used to detect Cy7-like dyes (eg, 780/60-nm filter).
|Pan Leukocyte, NHP-specific|
|Rhesus peripheral whole blood|
|Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.|
|Brilliant Violet 786|
|Aqueous buffered solution containing BSA and ≤0.09% sodium azide.|
|Baboon, Cynomolgus, Rhesus|
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