CD45 Mouse, BUV395, Clone: D058-1283, BD
Mouse Monoclonal Antibody
Manufacturer: BD Biosciences 564099
D058-1283 is a CD45 monoclonal antibody specific for non-human primate leucocytes, developed using Rhesus peripheral whole blood as immunogen. It does not cross react with human leucocytes. This antibody reacts with Baboon, Rhesus and Cynomolgus Macaque leucocytes in a similar pattern as seen with CD45 binding to the Leukocyte Common Antigen on human cells. Immunophenotypic analysis shows that D058-1283 binds to lymphocytes, monocytes and granulocytes of non-human primate blood samples. This antibody is able to block the binding of monoclonal antibody TÜ116; a reported anti-human CD45 antibody that cross-reacts with nonhuman primate leucocytes. In Western blot analysis, the D058-1283 antibody identifies a band of approximately 180-200 kDa molecular weight .
The antibody was conjugated to BD Horizon BUV395 which is part of the BD Horizon Brilliant Ultraviolet family of dyes. This dye is optimal for multicolor flow cytometry because it has little to no spillover into other detectors. With an Ex Max at 348nm and an Em Max at 395nm, BD Horizon BUV395 can be excited with a 355nm laser and detected with a 379/28 filter.
|Pan Leukocyte, NHP-specific|
|Rhesus peripheral whole blood|
|Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.|
|Brilliant Ultraviolet 395|
|Aqueous buffered solution containing BSA and ≤0.09% sodium azide.|
|Baboon, Cynomolgus, Rhesus|
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