CD45 Rat anti-Mouse, Unlabeled, Clone: 30-F11, BD

Rat Monoclonal Antibody

Supplier:  BD Biosciences 550539

Catalog No. BDB550539

Description

The 30-F11 antibody reacts with both alloantigens and all isoforms of the CD45 leukocytes common antigen (LCA), also known as Ly-5 or T200, found on hematopoietic stem cells and all cells of hematopoietic origin, except erythrocytes. CD45 is a transmembrane glycoprotein which is expressed at high levels on the cell-surface, and its presence distinguishes leukocytes from non-hematopoietic cells. CD45 is a member of the Protein Tyrosine Phosphatase (PTP) family: Its intracellular (COOH-terminal) region contains two PTP catalytic domains, and the extracellular region is highly variable due to alternative splicing of exons 4, 5, and 6 (designated A, B, and C, respectively), plus differing levels of glycosylation. The CD45 isoforms detected in the mouse are cell type-, maturation-, and activation state-specific. The CD45 isoforms play complex roles in T-cell and B-cell antigen receptor signal transduction. This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

Specifications

• Antigen: CD45
• Classification: Monoclonal
• Concentration: 62.5μg/mL
• Description: Ptprc; CD45R; LCA; Leukocyte common antigen; T200; B220; Ly-5; Lyt-4
• Host Species: Rat
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C

• Applications: Flow Cytometry
• Clone: 30-F11
• Conjugate: Unconjugated
• Formulation: Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.
• Immunogen: Mouse Thymus / Spleen
• Quantity: 1 mL
• Research Discipline: Immunology
• Target Species: Mouse
• Isotype: IgG2b κ

For Research Use Only.