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Description
D058-1283 is a CD45 monoclonal antibody specific for non-human primate leucocytes, developed using Rhesus peripheral whole blood as immunogen. It does not cross react with human leucocytes. This antibody reacts with Baboon, Rhesus and Cynomolgus Macaque leucocytes in a similar pattern as seen with CD45 binding to the Leukocyte Common Antigen on human cells. Immunophenotypic analysis shows that D058-1283 binds to lymphocytes, monocytes and granulocytes of non-human primate blood samples. This antibody is able to block the binding of monoclonal antibody TÜ116; a reported anti-human CD45 antibody that cross-reacts with nonhuman primate leucocytes. In Western blot analysis, the D058-1283 antibody identifies a band of approximately 180-200 kDa molecular weight .
The antibody is conjugated to BD Horizon™ V450, which has been developed for use in multicolor flow cytometry experiments and is available exclusively from BD Biosciences. It is excited by the Violet laser Ex max of 406nm and has an Em Max at 450 nm. Conjugates with BD Horizon™ V450 can be used in place of Pacific Blue™ conjugates.
Specifications
Specifications
| Antigen | CD45 |
| Applications | Flow Cytometry |
| Classification | Monoclonal |
| Clone | D058-1283 |
| Conjugate | Violet Fluor 450 (V450) |
| Description | Pan Leukocyte, NHP-specific |
| Formulation | Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide. |
| Host Species | Mouse |
| Immunogen | Rhesus peripheral whole blood |
| Purification Method | Affinity Purified |
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Safety and Handling
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