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CD45 Mouse anti-Human, V500, Clone: HI30, BD

Catalog No. BDB560779 Shop All BD Biosciences Products
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BDB560777 100 Tests
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Description

Mouse Monoclonal Antibody

Reacts with the 180, 190, 205, 220kDa isoforms of the leukocyte common antigen (LCA) present on all human leukocytes including lymphocytes, monocytes, granulocytes, eosinophils, and thymocytes but absent from circulating erythrocytes, platelets, or mature erythroid cells of bone marrow and non-hemopoietic tissues. The antibody is conjugated to BD Horizon™ V500, which has been developed for use in multicolor flow cytometry experiments and is available exclusively from BD Biosciences. It is excited by the Violet laser with an Ex max of 415nm and Em Max at 500nm. BD Horizon V500 conjugates emit at a similar wavelength to Amcyan yet exhibit reduced spillover into the FITC channel. For more information on BD Horizon V500, visit bdbiosciences.com/colors. When compensating dyes in this spectral range (such as Horizon™ V500 and AmCyan), the most accurate compensation can be obtained using single stained cellular controls. Due to spectral differences between cells and beads in this channel, using BD CompBeads can result in spillover errors for V500 and AmCyan reagents. Therefore, the use of BD CompBeads or BD CompBeads Plus to determine spillover values for these reagents is not recommended. Different V500 reagents (e.g. CD4 vs. CD45) can have slightly different fluorescence spillover therefore, it may also be necessary to use clone specific compensation controls when using these reagents.

Flow Cytometry

TRUSTED_SUSTAINABILITY

Specifications

Antigen CD45
Applications Flow Cytometry
Classification Monoclonal
Clone HI30
Conjugate Violet Fluor 500 (V500)
Formulation Aqueous buffered solution containing protein stabilizer, glycerol and ≤0.09% sodium azide.
Host Species Mouse
Immunogen Human Peripheral Blood Leucocytes
Purification Method Affinity Purified
Quantity 25 Tests
Regulatory Status RUO
Research Discipline Immunology
Primary or Secondary Primary
Target Species Human
Content And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Isotype IgG1 κ
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For Research Use Only.

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