MilliporeSigma™ anti-CD45R (B220) Red Fluor 710, Clone: RA3-6B2,

Rat Monoclonal Antibody

Supplier:  MilliporeSigma™ MABF1474

Catalog No. MABF1474MI

Description

Specifically detects CD45R (B220) Clone: RA3-6B2 in Human, Mouse samples, and it is validated for Flow Cytometry

The RA3-6B2 antibody reacts with the human and mouse CD45 isoform known as CD45R, or B220, a protein tyrosine phosphatase of 220kDa. CD45 is one of the most abundant hematopoietic markers, and is expressed on all leukocytes (the Leukocyte Common Antigen, LCA). Various isoforms are generated and expressed in cell-specific patterns, all critical for leukocyte function. In mouse, the CD45R/B220 isoform is predominantly found on B cells, at varying levels on all stages from pro-B cells to activated B cells, and may also be detected on certain T cell and NK cell subsets. It is of note that B220 is not similarly expressed on human B cells, where it appears to be differentiation-specific and therefore expressed on only some B cell subsets. Other forms of CD45 with restricted cellular expression include CD45RA, CD45RB, CD45RO and several others.The RA3-6B2 antibody is one of the most consistently used leukocyte markers for B cells, T cell subsets and NK cell subsets in human and mouse. This antibody has also been reported as cross-reactive with feline CD45R/B220.

Specifications

• Antigen: CD45R (B220)
• Classification: Monoclonal
• Conjugate: Red Fluor 710
• Gene Accession No.: P06800
• Host Species: Rat
• Purification Method: Purified by size-exclusion chromatography
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Content And Storage: 2°C to 8°C undiluted for one year from date of shipment protected from light; Do not freeze

• Applications: Flow Cytometry
• Clone: RA3-6B2
• Formulation: Purified Rat monoclonal containing 10mM NaH2PO4, 150mM NaCl, 0.09% NaN3, 0.1% gelatin, pH 7.2.
• Gene Symbols: Ptprc, Ly-5
• Immunogen: Proprietary immunogen
• Quantity: 100 μg
• Research Discipline: Inflammation, Immunology
• Target Species: Human, Mouse
• Form: Purified

For Research Use Only