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CD45RA Mouse anti-Human, Brilliant Violet 711, Clone: HI100, BD

Mouse Monoclonal Antibody

Manufacturer:  BD Biosciences 563733

Catalog No. BDB563733


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Description

Description

The HI100 monoclonal antibody specifically binds to the 220 kDa isoform of the human leukocyte common antigen, CD45RA. CD45RA is expressed on approximately 40-50% of peripheral CD4+ T cells, 50% of peripheral CD8+ T cells and on a portion of B cells and monocytes. The CD45RA antigen is expressed by naοve and activated T cells. CD45RA-specific antibodies are useful for the study of the suppressor/inducer subpopulation of CD4+ lymphocytes.

The antibody was conjugated to BD Horizon™ BV711 which is part of the BD Horizon™ Brilliant Violet™ family of dyes. This dye is a tandem fluorochrome of BD Horizon™ BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 711-nm. BD Horizon™ BV711 can be excited by the violet laser and detected in a filter used to detect Cy™5.5 / Alexa Fluor™ 700-like dyes (eg, 712/20-nm filter). Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy™5.5 detectors. However, the spillover can be corrected through compensation as with any other dye combination.

Specifications

Specifications

CD45RA
HI100
CD45R; PTPRC; LCA; Leukocyte common antigen
Affinity Purified
IgG2b, κ
RUO
Primary
Human
Flow Cytometry
Brilliant Violet 711
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Murine
50 Tests
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Monoclonal
Documents
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